Moreover, it became obvious that bilirubin administration could ameliorate structural changes in the rats livers receiving HFD
with elevator, no close family to help on regular basis
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LCMS/MS analysis was performed using an Advance nanoLC (BrukerMichrom, Auburn, CA, USA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific) equipped with a NANOHPLC capillary column C18 (0.075 mm ID 150 mm length, 3 m particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 535% CH 3 CN /0.1% formic acid) at a flow rate of 300 nL/min
The reason for this variation is still unknown but may be associated with mutations in the tumor suppressor gene TP53