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The eluted protein was concentrated to approximately 5 ml (Millipore Amicon 10 kDa molecular weight cut-off (MWCO)) and buffer-exchanged into storage buffer (25 mM HEPES pH 7.5 and 150 mM NaCl)

Hydroxocobalamin is the preferred B12 formulation in the UK due to its longer duration of action compared to cyanocobalamin

Biochem J 97:318320

Oral supplements, B-complex tablets, multivitamins, fortified cereals and plant milks, energy drinks and, above all, B12 injections all push the number up

Ramucirumab plus erlotinib in patients with untreated, EGFR-mutated, advanced non-small-cell lung cancer (RELAY): a randomised, double-blind, placebo-controlled, phase 3 trial