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In the transwell assay, a confluent monolayer of endothelial cells on a porous membrane separates an apical and basolateral chamber
Shaking creates aggressive mechanical forces that can break the delicate peptide bonds and denature the molecule, rendering it inactive
Grabe M, Oster G (2001) Regulation of organelle acidity
doi: 10.1038/nrn.2017.113 13 KlomjaiWKatzRLackmy-ValleA
181,182 The brain accumulation of electro-responsive nanogels was significantly improved by the functionalization
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