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Protein purification Cell pellets were resuspended in ice-cold lysis buffer containing 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, 0.2 mM PMSF, 20 g/mL DNase I, and 20 M DNQX
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Error 7: Continuous high doses without breaks The problem: Months of continuous high-dose injections can lead to tolerance development
Once approved, manufacturers are required to report any adverse events and monitor ongoing safety
DOI:10.1371/journal.pone.0118897 [ Links ] 57
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