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Step 6: Filter if required For cell culture applications, pass the solution through a 0.22 m syringe filter under sterile conditions to remove any particulates and ensure sterility
This becomes more relevant with extended use, typically after several months
Maintenance: 1 session every 34 weeks after completing the full course
PET scans are less useful for monitoring primary brain tumors or tumors that have spread to the brain from elsewhere in the body because the brain itself takes up a lot of glucose
To one medium, 16 L of Sodium Hydrogen L (+)-Glutamate monohydrate (Wako) dissolved in sterile water was added at final concentrations of 1, 5, 20, and 40 mM, and to the other medium, only 16 L of sterile water was added
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