To isolate intestinal organoid, following euthanasia of 810-week-old male mice, crypts were isolated from the ileum fragments by EDTA incubation and plated in Matrigel using advanced DMEM/F12 containing 100 U/mL penicillin-streptomycin, 10 mM HEPES, 2 mM GlutaMAX (35050061), supplements N2 (17502048) and B27 (17504044) from ThermoFisher, 50 ng/mL murine EGF (315-09, Peprotech), 1 mmol/L N-acetylcysteine (A9165, Sigma), 100 ng/mL murine Noggin (250-38, Peprotech), and 1000 ng/mL human R-spondin-1 (120-38-20, Peprotech)
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Diverticulosis is the result of weak areas within the colon wall and high pressure within the colon leading to small pockets that develop along the wall of the colon, most commonly the sigmoid colon
Kidney lipid dysmetabolism and lipid droplet accumulation in chronic kidney disease
[9] Therapy-resistant cancer cells in a high-mesenchymal state depend on a lipid peroxidase pathway to suppress ferroptosis, indicating a critical survival mechanism in this cellular context