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How many undiagnosed B12 deficient mothers find themselves devastated by post natal depression following nitrous oxide administration during labour
The alkylation of proteins was carried out by adding chloroacetamide (Sigma-Aldrich, St-Louis, MO, USA) to a final concentration of 7.5 mM followed by a 20-min incubation at room temperature protected from light
T.EklandE
For longer-term storage, freezing is standard practice for lyophilized peptides
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