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The initial inflammatory modulation begins within the first one to two weeks, but the functional changes, including improved flexibility, reduced global stiffness, and better circulation, accumulate over four to eight weeks as the compounds systemic effects build through repeated dosing

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The functionality of GST-GFP protein was confirmed by its increased affinity to nickel (data not shown) and glutathione beads, its green color and fluorescence, and its cleavability by thrombin