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Amino acid-dependent control of mTORC1 signaling: a variety of regulatory modes
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96 G Protein G proteins regulate scaffold proteins responsible for the cytoarchitecture of the TJ and membrane-associated proteins regulating TJ activity
Briefly, INS-1E cells were maintained in RPMI 1640 medium (Gibco) supplemented with 5% (v/v) heat-inactivated fetal bovine serum, 2 mM l-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/mL penicillin/streptomycin and 50 M 2-mercaptoethanol
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