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Presence, activity and properties of glutaredoxins are often analysed in coupled spectrophotometric reductive assays with bis(2-hydroxyethyl)disulfide (HEDS) as a non-glutathione substrate 10,11,12,15,16,17,18 or L-cysteine-glutathione disulfide (GSSCys) as a glutathionylated substrate 10,11,12,18,19,20,21 (Fig