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Plasmid for single-stranded adeno-associated viral vectors 2/8 The plasmid pAAV-MCS-hAAT/EGFP was designed for maximum transgene expression and includes the hepatic locus control region from the ApoE gene (ApoE-HCR), a liver-specific 1-antitrypsin promoter (hAAT promoter), coagulation factor IX intron A (FIX intron A), a 3' mRNA transcription termination/polyadenylation signal (bghpA), and a reporter gene encoding enhanced green fluorescent protein (EGFP), as previously described (Miao et al

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Notably, the deletion of Slc7a11 (which encodes xCT) in animals results in viable offspring 15 , which suggests that tissues (and potentially tumours) can obtain cysteine from another origin

Rajagopalan, S., Andreeva, A., Teufel, D