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J Biol Chem Elsevier
Temperature fluctuations, freeze-thaw cycles, shaking, and high concentrations all promote aggregation
However, as with any experimental treatment, potential risks and side effects may exist
Microanalysis 23, 11501158
In addition, the high hydrophilicity and small size of the Flag-tag have little effect on protein function, expression, maturation, and antigenicity
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