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A capillary C 18 trap column (3 cm 100 m) was used to loaded samples and then separate them by a C 18 column (15 cm 75 m) on an Eksigent nanoLC-1D plus system (SCIEX, USA)

Additionally, granulosa cells in women with endometriosis exhibit apoptosis, mitochondrial dysfunction, disrupted steroid hormone production, and increased inflammatory markers, further compromising follicle health (15)

The benefits build gradually, so consistency is key

All the experiments were repeated 3 times and the values represent the mean of triplicates and these values are given in the Fig

Cell Signaling: NAD+ participates in signaling pathways that regulate cell survival, aging, and stress responses