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Depending on the plate layout, either LPS (1 g/ml) or ConA, (2.5 g/ml, Sigma-Aldrich, Steinheim, Germany) was added in four replicates per cow in addition to four unstimulated wells (with Seahorse medium for volume compensation)

This will serve as a continued proof of concept to visually detect biological analytes

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Singh RB, Niaz MA, Agarwal P, Beegum R, Rastogi SS, Sachan DS