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Presented for research literature review only Khaw SC et al

The availability of Replenza GLP-1 Support can fluctuate, potentially leading to difficulties in obtaining it regularly

Mean weight loss was 4.5%, 9.2%, and 10.6% respectively, versus 2.0% with placebo

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After washing and resuspension in HBSS containing 0.1% BSA and metabolic inhibitors (20 mmol/L 2-deoxygucose and 10 mmol/L NaN 3 ) to prevent GLP-1R internalisation (Widmann et al., 1995), binding experiments were performed using FITC-conjugated ligands as described below, with TR-FRET measured in a Flexstation 3 plate reader (Molecular Devices) using the following settings: ex = 335 nm, em = 520 and 620 nm, delay 50 s, integration time 400 s