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Unfused albumin variants or GLP-1 albumin fusions (2 mg/mL) were loaded onto the column in a pH gradient from pH 5.5 to pH 8.8 by injecting a gradual mixture of 20 mM MES/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 5.5) and 20 mM Tris/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 8.8) at a flow rate of 0.5 mL/min for 110 min at room temperature

Ethics declarations Conflict of interest KE has been member on advisory boards for Eli Lilly & Co., NovoNordisk, Lifescan, Roche, GSK, Bayer and has received honoraria from these activities

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Journal of Agricultural and Food Chemistry , 65 , 92179225