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When the TSA method was used, the sections were treated with biotinylated goat anti-mouse/rabbit IgG (1:1000 dilution, Vector Laboratories, Burlingame, CA) for 1 h, and then incubated with HRP-conjugated-avidin for 30 min (1:100 dilution in TN buffer, TSA System) at room temperature
Others prescribe the stack from day one for patients specifically focused on body composition
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GIP-R are distributed in the pancreas but also in the heart, the pituitary, the adrenal cortex, some areas of the central nervous system (CNS) and both brown and white adipose tissues where it promotes fat deposition (8)