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The protein sample (250 g protein /30 L) was mixed with 270 L of UA (100 mM TrisHCl (pH 8.5) containing 8 M Urea) in the filter unit, and centrifuged at 14,000 g for 15 min
Overall, the observed patterns were primarily related to amino acid metabolism, lipid metabolism, and neurotransmitter-associated pathways, suggesting pathway-level metabolic alterations in ASD
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