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Suppliers who cannot or do not provide these documents for both compounds in this stack create unnecessary uncertainty about whether any protocol results are reflecting the compounds true pharmacological properties
There is also a decline of IL-2, as well as chemotaxis, phagocytosis and adequate levels of ROS in the phagosomes
The proteins were eluted with 0.2 mg/ml FLAG-peptide in 20 mM HEPES pH 7.5, 100 mM NaCl buffer, then mixed with Laemmli sample buffer (BioRad), subjected to SDS-PAGE and Western blotting and detected by monoclonal ANTI-FLAG M2-peroxidase (HRP) antibody (1:2000) (A8592, Sigma-Aldrich, RRID: AB_439702) for SH3-FLAG and polyclonal A1CT antibody 58 (1:5000) and HRP-conjugated ECL Rabbit IgG (1:5000) (NA9340, Amersham, RRID:AB_772191) for arr1
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A selective inhibitor Gal-PUGNAc of human lysosomal beta-hexosaminidases modulates levels of the ganglioside GM2 in neuroblastoma cells
