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The supernatants, so-called post-nuclear supernatant (PNS) were taken in Eppendorf and centrifuged at 4 C for 10 min at 10,000 g
They reflect the accelerated rate at which microbial activity increases at higher temperatures
in 1980
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This qualitative improvement matters tremendously for preventing the recurrent sprains and chronic instability that plague many individuals after initial ankle injuries
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