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The urine samples were centrifuged at 4C, 1,000 g for 20 min to obtain the supernatant and the concentrations of urinary albumin and creatinine were determined using a mouse Mau ELISA kit (Wuhan Elabscience Biotechnology Co., Ltd

They are not approved by the FDA or any other regulatory body for human or animal use, consumption, or therapeutic application

Y2H and GST pull-down assays showed that Pelo interacted with an E3 ubiquitin ligase, encoding a 658 amino acid protein that contained a C3HC4-type RING domain at its N-terminus (Fig

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