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Dominant negative G s (DNG s ) in pFastBac vector, and poly-his tagged G1 and G2 in a single pVL dual vector construct were used as previously described

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In order to monitor the intracellular translocation of agonists, as well as GLP-1Rs, we used fluorescently labeled exendin-4, exendin-phe1, and exendin-asp3 conjugates, with fluorescein isothiocyanate (FITC) at position K12, which we previously found useful for labeling GLP-1R 28