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doi: 10.1016/j.eclinm.2024.102789 28
Acute liraglutide attenuated the alcohol-induced increase in accumbal dopamine, decreased alcohol-induced CPP, and prevented alcohol deprivation-induced drinking
Week 24 What you may notice: Clothes may start fitting differently
After washing two times with M9 buffer (3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , 5 g NaCl, 1 ml 1 M MgSO 4 , H 2 O to 1 litre), whole worms were fixed with 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 20 min, and then post-fixed with cold 100% methanol for 5 min at 20C (alternatively, fixed worms can be stored in cold methanol at -20C for a few days)
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