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Cells were lysed in 1 mL of lysis buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 1% NP40, 0.5% Triton X-100, and 10% glycerol) supplemented with 20 M PR619, 10 mM iodoacetamide, and a protease inhibitor cocktail and were sonicated using Handy Sonic (TOMY Seiko)

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