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10.1016/j.biopsych.2010.03.022 249
The sections were blocked for 1 h at room temperature with 5% normal goat serum and stained with anti-atrial natriuretic peptide (ANP) antibody (Servicebio, Wuhan, China) diluted 1: 200, anti-myosin heavy chain 7(MYH7) antibody (Proteintech Group, Inc, Wuhan, China) diluted 1: 200, anti-cytochrome c oxidase subunit II (COX II) antibody (Cell Signaling Technology, Inc, Boston, USA) diluted 1: 1000, anti-NLRP3 antibody diluted (ABclonal, Wuhan, China) 1: 200, and anti- interleukin-18 (IL-18) antibody (Servicebio, Wuhan, China) diluted 1: 1000 overnight at 4 C
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