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Western blot assay K7M2 cells were incubated with the PBS, Ru(II)-OH, NP2, or NP4 (10 M Ru) for 12 h at 37 C, and the supernatant was subsequently co-incubated with RAW264.7 cells
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Previous studies have demonstrated that the aqueous humor of glaucoma patients shows alterations in the levels of certain proteins, such as those that contribute to oxidative damage, mitochondrial damage, neural degeneration, apoptosis, immune responses, and the complement cascade 19,20,21,22
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