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The skin gets dull and loses its brightness as a result

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To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h

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At predetermined intervals (0.5, 1, 2, 4, 6, 8, 12, 24, and 48 h), 1 mL aliquots were withdrawn and replaced with fresh medium to maintain constant volume