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The in vitro experiments were performed using TM3 mouse Leydig cells that were cultured in DMEM/F-12 medium supplemented with 5% horse serum and 2.5% fetal bovine serum, and maintained at 37C under a 5% CO2 atmosphere
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The peptide normalized lower esophageal sphincter pressure in rat models of GERD, suggesting potential for addressing underlying pathophysiology rather than merely symptom suppression
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