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Many eukaryotic proteins are produced as inclusion bodies (insoluble aggregated protein), a malfunctioning protein caused by misfolding, in prokaryotic expression systems such as E

(2021) Thymosin 4 function review Frontiers in Endocrinology Sosne & Kleinman (2015) Tissue repair mechanisms IOVS/Arvo Journals Goldstein et al

Before experiment, cells were dissociated by trypsin (0.05%, Sigma Aldrich) and cell concentration measured using a Sceptor pipette (Millipore, Burlington, USA)

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S., Mayer, C., Platt, R