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Signs of bacterial contamination: Cloudiness (should be clear) Particles or floating material Unusual odor Color change Physical degradation signs Visual changes indicating degradation: Clear solution becomes cloudy Color change (especially copper peptides) Precipitation or crystals forming Separation of layers When in doubt: Discard

Continued use maintains results, but consistency is essential

Global disruption of Gclm results in mice (GCLM knockout) that have 15% of hepatic GSH levels seen in wild-type mice 16

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doi: 10.1016/j.isci.2021.102837