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Quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) Total RNA was isolated with Superbrilliant 6 min High-quality RNA Extraction Kit (zsgentech, Tianjin, China), according to the manufacturer's instructions

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Briefly, the islets were incubated in RPMI 1640 Medium (Gibco) at 37 C overnight, before they were washed in Krebs-Ringer HEPES (KRH) solution with 2.8 mM glucose (low-glucose buffer)