The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2
Nuclear factor-B The nuclear factor-B (NF-B) is an important inducible transcription factor playing a key role in processes of the immune and inflammatory responses, cellular adhesion, differentiation, proliferation, and apoptosis (Bonizzi and Karin 2004)
Additionally, Schwann cell () nuclei and axon structures were histologically normal
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